A Novel Strategy for the Detection of SARS-CoV-2 Variants Based on Multiplex PCR-Mass Spectrometry Minisequencing Technology

作者：Fei Zhao, Jinxing Lu, Bing Lu, Tian Qin, Xuemei Wang, Xuexin Hou, Fanliang Meng, Xiaona Xu, Tianyi Li, Haijian Zhou, Jianzhong Zhang, Biao Kan, Ying Huang, Zehui Zhang, Di Xiao · 发表于：Microbiology Spectrum · 年份：2021 · DOI：10.1128/spectrum.01267-21 · 被引用次数：45 · 研究领域：SARS-CoV-2 detection and testing、SARS-CoV-2 and COVID-19 Research、Biosensors and Analytical Detection

The emergence of SARS-CoV-2 variants is the key factor in the second wave of the COVID-19 pandemic. An all-in-one SARS-CoV-2 variant identification method based on a multiplex PCR-mass spectrometry minisequencing system was developed in this study. Six SARS-CoV-2 variants (Alpha, Beta, Epsilon, Iota, Gamma, and Delta) can be identified simultaneously. This method can not only achieve the multisite simultaneous detection that cannot be realized by PCR coupled with first-generation sequencing technology and quantitative PCR (qPCR) technology but also avoid the shortcomings of time-consuming, high-cost, and high technical requirements of whole-genome sequencing technology. As a simple screening assay for monitoring the emergence and spread of SARS-CoV-2 and variants, mPCR-MS minisequencing is expected to play an important role in the detection and monitoring of SARS-CoV-2 infection as a supplementary technology.