[Effect of Echinococcus multilocularis infections on mitochondrial functions of macrophages].

作者：Jinxin Deng, Dengliang Huang, Yinhang Zhang, Jianhui Li, Jing Hou, Yunpeng Jiang, Mingqian Tian, Lin Sun, Tianlun Zhang, X Zhang, Yan Dong, Haining Fan, Y Y · 发表于：PubMed · 年份：2021 · DOI：10.16250/j.32.1374.2021066 · 研究领域：Parasitic infections in humans and animals、Hydrogen's biological and therapeutic effects、Amoebic Infections and Treatments

OBJECTIVE: infections, so as to provide insights into the pathogenesis of alveolar echinococcosis. METHODS: Deep Red FM and the mean fluorescence intensity of macrophage mitochondria was measured with the Cytation 5 Cell Imaging Multi-Mode Reader. The mitochondrial DNA copy number was quantified using the quantitative real-time PCR (qPCR) assay, and the mitochondrial energy metabolism was monitored using the Seahorse XF assay. In addition, the mitochondrial reactive oxygen species and mitochondrial membrane potential were detected using flow cytometry. RESULTS: < 0.01) in the 72 h culture group than in the control group. CONCLUSIONS: infection may impair mitochondrial functions and inhibit oxidative phosphorylation of macrophages, resulting in increased macrophage glycolysis. It is speculated that the alteration of macrophage metabolic states may contribute to the mechanisms underlying the development and progression of alveolar echinococcosis.