Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

作者：Jiaqiang Wu, Haoyu Lang, Xiaohuan Mu, Zijing Zhang, Qinzhi Su, Xiaosong Hu, Hao Zheng · 发表于：Microbiome · 年份：2021 · DOI：10.1186/s40168-021-01174-y · 被引用次数：79 · 研究领域：Insect and Pesticide Research、Insect symbiosis and bacterial influences、Insect and Arachnid Ecology and Behavior

BACKGROUND: Honey bee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have been detected across honey bees. Although the bee gut microbiota structure is influenced by environmental factors, the heritability of the gut members and the contribution of the host genetics remains elusive. Considering bees within a colony are not readily genetically identical due to the polyandry of the queen, we hypothesize that the microbiota structure can be shaped by host genetics. RESULTS: We used shotgun metagenomics to simultaneously profile the microbiota and host genotypes of bees from hives of four different subspecies. Gut composition is more distant between genetically different bees at both phylotype- and "sequence-discrete population" levels. We then performed a successive passaging experiment within colonies of hybrid bees generated by artificial insemination, which revealed that the microbial composition dramatically shifts across batches of bees during the social transmission. Specifically, different strains from the phylotype of Snodgrassella alvi are preferentially selected by genetically varied hosts, and strains from different hosts show a remarkably biased distribution of single-nucleotide polymorphism in the Type IV pili loci. Genome-wide association analysis identified that the relative abundance of a cluster of Bifidobacterium st...