Melatonin supplementation in the culture medium rescues impaired glucose metabolism in IVF mice offspring

作者：Yanping Jia, Wenqiang Liu, Dandan Bai, Yalin Zhang, Yanhe Li, Yingdong Liu, Jiqing Yin, Qiaoyu Chen, Mingming Ye, Yanhong Zhao, Xiaochen Kou, Hong Wang, Shaorong Gao, Kunming Li, Miaoxin Chen · 发表于：Journal of Pineal Research · 年份：2021 · DOI：10.1111/jpi.12778 · 被引用次数：26 · 研究领域：Reproductive Biology and Fertility、Birth, Development, and Health、Assisted Reproductive Technology and Twin Pregnancy

Abstract Increasing evidence suggests that in vitro fertilization (IVF) may be associated with an increased risk of developing obesity and metabolic diseases later in life in the offspring. Notably, the addition of melatonin to culture medium may improve embryo development and prevent cardiovascular dysfunction in IVF adult mice. This study aimed to determine if melatonin supplementation in the culture medium can reverse impaired glucose metabolism in IVF mice offspring and the underlying mechanisms. Blastocysts used for transfer were generated by natural mating (control group) or IVF with or without melatonin (10−6 M) supplementation (mIVF and IVF group, respectively) in clinical‐grade culture media. Here, we first report that IVF decreased hepatic expression of Fbxl7, which was associated with impaired glucose metabolism in mice offspring. Melatonin addition reversed the phenotype by up‐regulating the expression of hepatic Fbxl7. In vitro experiments showed that Fbxl7 enhanced the insulin signaling pathway by degrading RhoA through ubiquitination and was up‐regulated by transcription factor Foxa2. Specific knockout of Fbxl7 in the liver of adult mice, through tail intravenous injection of recombinant adeno‐associated virus, impaired glucose tolerance, while overexpression of hepatic Fbxl7 significantly improved glucose tolerance in adult IVF mice. Thus, the data suggest that Fbxl7 plays an important role in maintaining glucose metabolism of mice, and melatonin supplementati...