Maintenance of genome sequence integrity in long- and short-lived rodent species

作者：Lei Zhang, Xiao Dong, Xiao Tian, Moonsook Lee, Julia Ablaeva, Denis Firsanov, Sang‐Goo Lee, Alexander Y. Maslov, Vadim N. Gladyshev, Andrei Seluanov, Vera Gorbunova, Jan Vijg · 发表于：Science Advances · 年份：2021 · DOI：10.1126/sciadv.abj3284 · 被引用次数：55 · 研究领域：Genomics and Phylogenetic Studies、Evolution and Genetic Dynamics、Mitochondrial Function and Pathology

DNA mutations in somatic cells have been implicated in the causation of aging, with longer-lived species having a higher capacity to maintain genome sequence integrity than shorter-lived species. In an attempt to directly test this hypothesis, we used single-cell whole-genome sequencing to analyze spontaneous and bleomycin-induced somatic mutations in lung fibroblasts of four rodent species with distinct maximum life spans, including mouse, guinea pig, blind mole-rat, and naked mole-rat, as well as humans. As predicted, the mutagen-induced mutation frequencies inversely correlated with species-specific maximum life span, with the greatest difference observed between the mouse and all other species. These results suggest that long-lived species are capable of processing DNA damage in a more accurate way than short-lived species.