Genome-Wide Identification of CBL-CIPK Gene Family in Honeysuckle (Lonicera japonica Thunb.) and Their Regulated Expression Under Salt Stress

作者：Luyao Huang, Zhuangzhuang Li, Qingxia Fu, Conglian Liang, Zhenhua Liu, Qian Liu, Gaobin Pu, Jia Li · 发表于：Frontiers in Genetics · 年份：2021 · DOI：10.3389/fgene.2021.751040 · 被引用次数：25 · 研究领域：Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms、Plant Molecular Biology Research

In plants, calcineurin B-like proteins (CBLs) are a unique group of Ca 2+ sensors that decode Ca 2+ signals by activating a family of plant-specific protein kinases known as CBL-interacting protein kinases (CIPKs). CBL-CIPK gene families and their interacting complexes are involved in regulating plant responses to various environmental stimuli. To gain insight into the functional divergence of CBL-CIPK genes in honeysuckle, a total of six LjCBL and 17 LjCIPK genes were identified. The phylogenetic analysis along with the gene structure analysis divided both CBL and CBL-interacting protein kinase genes into four subgroups and validated by the distribution of conserved protein motifs. The 3-D structure prediction of proteins shown that most LjCBLs shared the same Protein Data Bank hit 1uhnA and most LjCIPKs shared the 6c9Da. Analysis of cis-acting elements and gene ontology implied that both LjCBL and LjCIPK genes could be involved in hormone signal responsiveness and stress adaptation. Protein-protein interaction prediction suggested that LjCBL4 is hypothesized to interact with LjCIPK7 / 9 / 15 / 16 and SOS1 / NHX1 . Gene expression analysis in response to salinity stress revealed that LjCBL2 / 4 , LjCIPK1 / 15 / 17 under all treatments gradually increased over time until peak expression at 72 h. These results demonstrated the conservation of salt overly sensitive pathway genes in honeysuckle and a model of Ca 2+ - LjCBL4 / LjSOS3 - LjCIPK16 / LjSOS2 module-mediated salt stres...