Transmission and molecular characteristics of bla NDM-producing Escherichia coli between companion animals and their healthcare providers in Guangzhou, China

作者：Min-Ge Wang, Chang Fang, Kaidi Liu, Linlin Wang, Ruan-Yang Sun, Rong‐Min Zhang, Liang‐Xing Fang, Jian Sun, Ya-Hong Liu, Xiao‐Ping Liao · 发表于：Journal of Antimicrobial Chemotherapy · 年份：2021 · DOI：10.1093/jac/dkab382 · 被引用次数：24 · 研究领域：Antibiotic Resistance in Bacteria、Pneumonia and Respiratory Infections、Antibiotic Use and Resistance

OBJECTIVES: To determine the transmission and molecular characteristics of blaNDM-producing Escherichia coli between companion animals and their healthcare providers at veterinary clinics in Guangzhou, China. METHODS: A total of 359 samples from companion animals and their healthcare providers were collected at 14 veterinary clinics in Guangzhou, China. Genomic characteristics and clonal relationships for blaNDM-positive E. coli and complete plasmid sequences were characterized based on WGS data from combined Illumina and MinION platform reads. RESULTS: Forty-five blaNDM-positive bacteria were recovered from companion animals (n = 43) and their healthcare providers (n = 2) at 10 veterinary clinics. Overall, E. coli (73.3%, 33/45) and Klebsiella pneumoniae (13.3%, 6/45) were the most prevalent species among the seven species of blaNDM-positive bacteria. Four blaNDM variants (blaNDM-1, blaNDM-4, blaNDM-5 and blaNDM-7) were identified in 45 blaNDM-positive bacteria and blaNDM-5 was the most prevalent (77.8%, 35/45). WGS indicated that the most prevalent STs were ST405 (8/33), ST453 (6/33), ST457 (6/33) and ST410 (5/33) among the 33 blaNDM-positive E. coli isolates. Phylogenomics and PFGE analysis revealed that clonal spread of blaNDM-positive ST453 E. coli isolates between companion animals and their healthcare providers was evident. In addition, two novel IncFIB plasmids carrying blaNDM-4 (pF765_FIB and pG908_FIB) were found in this study and indicated that IS26 may promote the...