Nucleocytoplasmic Shuttling of Porcine Parvovirus NS1 Protein Mediated by the CRM1 Nuclear Export Pathway and the Importin α/β Nuclear Import Pathway

作者：Liyan Cao, Fang Fu, Jianfei Chen, Hongyan Shi, Xin Zhang, Jianbo Liu, Da Shi, Yong Huang, Dewen Tong, Li Feng · 发表于：Journal of Virology · 年份：2021 · DOI：10.1128/jvi.01481-21 · 被引用次数：19 · 研究领域：Nuclear Structure and Function、Virus-based gene therapy research、Animal Virus Infections Studies

PPV replicates in the nucleus, and the nuclear envelope is a barrier to its entry into and egress from the nucleus. PPV NS1 is a nucleus-targeting protein that is important for viral DNA replication. Because the NS1 molecule is large (>50 kDa), it cannot pass through the nuclear pore complex by diffusion alone and requires specific transport receptors to permit its nucleocytoplasmic shuttling. In this study, the two functional NESs in the NS1 protein were identified, and their dependence on the CRM1 pathway for nuclear export was demonstrated. The nuclear import of NS1 utilizes importins α5 and α7 in the importin α/β nuclear import pathway.