Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile

作者：Haoming Xu, Hongli Huang, Yan‐Di Liu, Jiaqi Zhu, Youlian Zhou, You-Lian Zhou, Huiting Chen, Jing Xu, Hailan Zhao, Xue Guo, Wei Shi, Yuqiang Nie, Yongjian Zhou, Yongjian Zhou · 发表于：BMC Microbiology · 年份：2021 · DOI：10.1186/s12866-021-02342-8 · 被引用次数：102 · 研究领域：Gut microbiota and health、Inflammatory Bowel Disease、Probiotics and Fermented Foods

BACKGROUND: Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic colitis. METHODS: Acute colitis was induced in mice by administering 3% DSS for 1 week in the drinking water, and chronic colitis was induced by supplementing drinking water with 2.5% DSS every other week for 5 weeks. Control groups received the same drinking water without DSS supplementation. The histopathological score and length of the colons, and disease activity index (DAI) were evaluated to confirm the presence of experimental colitis. Intestinal microbiota was profiled by 16S rDNA sequencing of cecal content. RESULTS: Mice with both acute and chronic DSS-triggered colitis had significantly higher DAI and colon histopathological scores in contrast to the control groups (P < 0.0001, P < 0.0001), and the colon was remarkably shortened (P < 0.0001, P < 0.0001). The gut microbiota α-diversity was partly downregulated in both acute and chronic colitis groups in contrast to their respective control groups (Pielou index P = 0.0022, P = 0.0649; Shannon index P = 0.0022, P = 0.0931). The reduction in the Pielou and Shannon indices were more obvious in mice with acute colitis (P = 0.0022, P = 0.0043). The relative abundance of Bacteroides and Turicibacter was increased (all P < 0.05), while that ...