[Retracted] Influence of miRNA‐30a‐5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin‐1

作者：Hanyu Liu, Yabo Li, Yingdong Zou, Xingzong Zhang, Xiongfei Shi, Zhiping Yin, Yun Lin · 发表于：BioMed Research International · 年份：2021 · DOI：10.1155/2021/9963700 · 被引用次数：8 · 研究领域：Autophagy in Disease and Therapy

The study is aimed at observing the influence of microribonucleic acid‐ (miRNA‐) 30a‐50p on the pulmonary fibrosis in mice with Streptococcus pneumoniae infection through the regulation of autophagy by Beclin‐1. Specific pathogen‐free mice were instilled with Streptococcus pneumoniae through the trachea to establish the pulmonary fibrosis model. Then, they were divided into the miRNA‐30a‐50p mimics group (mimics group, n = 10) and miRNA‐30a‐5p inhibitors group (inhibitors group, n = 10), with the control group ( n = 10) also set. Pulmonary tissue wet weight/dry weight (W/D) was detected. The content of tumor necrosis factor‐ α (TNF‐ α ), interleukin‐ (IL‐) 6, and myeloperoxidase (MPO) was determined using enzyme‐linked immunosorbent assay (ELISA). Besides, the changes in the pulmonary function index dynamic lung compliance (Cdyn), plateau pressure (Pplat), and peak airway pressure (Ppeak) were monitored, and the gene and protein expression levels were measured via quantitative PCR (qPCR) and Western blotting. The expression level of miRNA‐30a‐5p was substantially raised in the mimics group ( p < 0.05), but extremely low in the inhibitors group ( p < 0.05). The mimics group had obviously raised levels of serum aminotransferase (AST), glutamic‐pyruvic transaminase (GPT), alkaline phosphatase (ALP), and pulmonary tissue W/D ( p < 0.05). Additionally, the expression levels of TNF‐ α , IL‐6, and MPO were notably elevated in the mimics group, while their expression levels ...