eggNOG-mapper v2: Functional Annotation, Orthology Assignments, and Domain Prediction at the Metagenomic Scale

作者：Carlos P. Cantalapiedra, Ana Hernández-Plaza, Ivica Letunić, Peer Bork, Jaime Huerta‐Cepas · 发表于：Molecular Biology and Evolution · 年份：2021 · DOI：10.1093/molbev/msab293 · 被引用次数：4996 · 研究领域：Genomics and Phylogenetic Studies、Bioinformatics and Genomic Networks、Microbial Metabolic Engineering and Bioproduction

Even though automated functional annotation of genes represents a fundamental step in most genomic and metagenomic workflows, it remains challenging at large scales. Here, we describe a major upgrade to eggNOG-mapper, a tool for functional annotation based on precomputed orthology assignments, now optimized for vast (meta)genomic data sets. Improvements in version 2 include a full update of both the genomes and functional databases to those from eggNOG v5, as well as several efficiency enhancements and new features. Most notably, eggNOG-mapper v2 now allows for: 1) de novo gene prediction from raw contigs, 2) built-in pairwise orthology prediction, 3) fast protein domain discovery, and 4) automated GFF decoration. eggNOG-mapper v2 is available as a standalone tool or as an online service at http://eggnog-mapper.embl.de.