The Effect of Salt‐Tolerant Antagonistic Bacteria CZ‐6 on the Rhizosphere Microbial Community of Winter Jujube ( Ziziphus jujuba Mill. “Dongzao”) in Saline‐Alkali Land

作者：Yanyan Zhou, Liping Hao, Chao Ji, QiSheng Zhou, Xin Song, Yue Liu, Huying Li, Chaohui Li, Qixiong Gao, Jintai Li, Pengcheng Zhang, Xunli Liu · 发表于：BioMed Research International · 年份：2021 · DOI：10.1155/2021/5171086 · 被引用次数：23 · 研究领域：Plant-Microbe Interactions and Immunity、Legume Nitrogen Fixing Symbiosis、Polysaccharides and Plant Cell Walls

As the main economic crop cultivated in the Yellow River Delta, winter jujube contains various nutrients. However, soil salinization and fungal diseases have affected the yield and quality of winter jujube. In order to use plant growth‐promoting rhizobacteria (PGPR) to reduce these damages, the antagonistic bacteria CZ‐6 isolated from the rhizosphere of wheat in saline soil was selected for experiment. Gene sequencing analysis identified CZ‐6 as Bacillus amyloliquefaciens . In order to understand the salt tolerant and disease‐resistant effects of CZ‐6 strain, determination of related indicators of salt tolerance, pathogen antagonistic tests, and anti‐fungal mechanism analyses was carried out. A pot experiment was conducted to evaluate the effect of CZ‐6 inoculation on the rhizosphere microbial community of winter jujube. The salt tolerance test showed that CZ‐6 strain can survive in a medium with a NaCl concentration of 10% and produces indole acetic acid (IAA) and 1‐aminocyclopropane‐1‐carboxylic acid (ACC) deaminase. Studies on the inhibition mechanism of pathogenic fungi show that CZ‐6 can secrete cellulase, protease, and xylanase. Gas chromatography‐mass spectrometry (GC‐MS) analysis showed that CZ‐6 can release volatile organic compounds (VOCs), including 2‐heptanone and 2‐nonanone. In addition, the strain can colonize the rhizosphere and migrate to the roots, stems, and leaves of winter jujube, which is essential for plant growth or defense against pathogens. Illumina M...