Clinical Molecular and Genomic Epidemiology of Morganella morganii in China

作者：Guoxiu Xiang, Kai Lan, Yimei Cai, Kang Liao, Mei Zhao, Jia Tao, Yi Ma, Jianming Zeng, Weizheng Zhang, Zhongwen Wu, Xuegao Yu, Yuyang Liu, Yang Lü, Caixia Xu, Liang Chen, Yi‐Wei Tang, Chen Cha, Wei Jia, Bin Huang · 发表于：Frontiers in Microbiology · 年份：2021 · DOI：10.3389/fmicb.2021.744291 · 被引用次数：34 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial Identification and Susceptibility Testing、Antimicrobial Resistance in Staphylococcus

Objectives: Ongoing acquisition of antimicrobial resistance genes has made Morganella morganii a new clinical treatment challenge. Understanding the molecular epidemiology of M. morganii will contribute to clinical treatment and prevention. Methods: We undertook a 6-year clinical molecular epidemiological investigation of M. morganii from three tertiary hospitals in China since 2014. Antimicrobial susceptibility testing was performed using a VITEK-2 system. All isolates were screened for β-lactam and plasmid-mediated quinolone resistance genes by PCR. Isolates carrying carbapenem-resistant genes were subjected to whole-genome sequencing (WGS). The variation and evolution of these mobile genetic elements (MGEs) were then systematically analyzed. Results: Among all M. morganii isolates ( n = 335), forty (11.9%) were recognized as multidrug resistant strains. qnrD1 , aac(6′)-Ib-cr , bla TEM–104 , and bla CTX–M–162 were the top four most prevalent resistance genes. Notably, phylogenomic and population structure analysis suggested clade 1 (rhierBAPS SC3 and SC5) associated with multiple resistance genes seemed to be widely spread. WGS showed a bla OXA–181 -carrying IncX3 plasmid and a Proteus genomic island 2 variant carrying bla CTX–M–3 , aac(6′)-Ib-cr coexisted in the same multidrug resistant strain zy_m28. Additionally, a bla IMP–1 -carrying IncP-1β type plasmid was found in the strain nx_m63. Conclusion: This study indicates a clade of M. morganii is prone to acquire resistanc...