Atractylodin may induce ferroptosis of human hepatocellular carcinoma cells

作者：Yongfei He, Dalang Fang, Tianyi Liang, Hongbing Pang, Yingdan Nong, Li-Bo Tang, Ziye Yang, Chunmiao Lu, Xiao Han, Shuqi Zhao, Shutian Mo, Yuhua Meng, Chuangye Han, Tao Peng · 发表于：Annals of Translational Medicine · 年份：2021 · DOI：10.21037/atm-21-4386 · 被引用次数：37 · 研究领域：Ferroptosis and cancer prognosis、Cancer-related molecular mechanisms research、Immune cells in cancer

BACKGROUND: It has been reported that atractylodin has a potential antitumor effect. This study aimed to investigate the effects of atractylodin on Huh7 and Hccm hepatocellular carcinoma (HCC) cells and its molecular mechanism. METHODS: , and their viability was detected by CCK-8 assay and the half inhibitory concentration (IC50) was calculated. The cells were treated with different concentrations of atractylodin, and the migration and invasion ability of cells was detected by scratch assay and Transwell assay. The cell cycle change and apoptosis rate were detected by flow cytometry. IlluminaHiSeq4000 platform was used for transcriptome sequencing, and the results were analyzed for gene differential expression, gene function, and signal pathway enrichment. Morphological changes of cells were detected by transmission electron microscopy, reactive oxygen species (ROS) levels were detected by DCFH-DA probe, and the expressions of ferroptosis related proteins GPX4, ACSL4, FTL, and TFR1 were detected by Western blot. RESULTS: The results showed that atractylodin could inhibit the proliferation, migration, and invasion of Huh7 and Hccm cells, regulate the cell cycle, and induce cell apoptosis and G1 phase cell cycle arrest. In addition, it could significantly induce the increase of intracellular ROS levels, decrease the expression of GPX4 and FTL proteins, and up-regulate the expression of ACSL4 and TFR1 proteins. CONCLUSIONS: Atractylodin can inhibit the proliferation, migration, ...