Blood small extracellular vesicles derived miRNAs to differentiate pancreatic ductal adenocarcinoma from chronic pancreatitis

作者：Shiwei Guo, Hao Qin, Ke Liu, Huan Wang, Sijia Bai, Shiyi Liu, Zhuo Shao, Yanan Zhang, Bin Song, Xiaoya Xu, Jing Shen, Peng Zeng, Xiaohan Shi, Hao Chen, Suizhi Gao, Jiajia Xu, Yaqi Pan, Lei Xiong, Fugen Li, Dadong Zhang, Xiao‐Dong Jiao, Gang Jin · 发表于：Clinical and Translational Medicine · 年份：2021 · DOI：10.1002/ctm2.520 · 被引用次数：29 · 研究领域：Extracellular vesicles in disease、Pancreatic and Hepatic Oncology Research、Ferroptosis and cancer prognosis

BACKGROUND: The differential diagnosis of pancreatic ductal adenocarcinoma (PDAC) from chronic pancreatitis (CP) is clinically challenging due to a lack of minimally invasive diagnosis methods. MicroRNAs (miRNAs) derived from small extracellular vesicles (EVs) in the blood have been reported as a promising diagnosis biomarker for various types of cancer. However, blood small EV miRNA signatures and their diagnostic value to differentiate between PDAC and CP remain to be determined. METHODS: In this study, 107 patients with PDAC or CP were recruited, and 90 patients were finally enrolled for a training cohort (n = 48) and test cohort (n = 42). Small RNA sequencing was used to assess the expression of blood small EV miRNAs in these patients. RESULTS: The linear model from the differentially expressed blood small EV miR-95-3p divided by miR-26b-5p showed an average sensitivity of 84.1% and an average specificity of 96.6% to identify PDAC from CP in the training cohort and the test cohort, respectively. When the model was combined with serum carbohydrate antigen 19-9 (CA19-9), the average sensitivity increased to 96.5%, and the average specificity remained at 96.4% of both cohorts, which demonstrated the best performance of all the published biomarkers for distinguishing between PDAC and CP. The causal analysis performed using the Bayesian network demonstrated that miR-95-3p was associated with a "consequence" of "cancer" and miR-26b-5p as a "cause" of "pancreatitis." A subgroup ...