Composition and antifungal mechanism of essential oil from Chrysanthemum morifolium cv. Fubaiju

作者：Jianfeng Zhan, Feng He, Huimin Cai, Meichan Wu, Yunli Xiao, Xiang Fu, Ying Yang, Chen Ye, Shuzheng Wang, Shiming Li · 发表于：Journal of Functional Foods · 年份：2021 · DOI：10.1016/j.jff.2021.104746 · 被引用次数：44 · 研究领域：Essential Oils and Antimicrobial Activity、Phytochemistry and Biological Activities、Natural product bioactivities and synthesis

Chrysanthemum morifolium cv. Fubaiju (CMF) has been used as a dietary herb in East Asia for centuries. The chemical composition and antifungal activity of CMF essential oil (CMF-EO) were investigated in this study. We have found that CMF-EO was a light-yellow, transparent liquid (0.61% yield, w/w) and majorly contain 31 volatile components with a total of 98.41% determined by GS and GC/MS. Camphor was the major compound (16.87%), followed by 1,8-cineole (10.62%), β-curcumene (9.51%), bornyl acetate (7.43%), α-zingiberene (6.97%), β-elemene (6.47%), α-bisabolol (4.74%), borneol (4.24%) and eugenol (4.23%). In addition, contents with more than 2% of the total CMF-EO were 1-caryophyllene (3.92%), chrysanthenyl acetate (3.29%), caryophyllene oxide (2.28%), and chamazulene (2.23%). Six fungal strains were used to study the antifungal activities and mechanisms of CMF-EO using fluorescence probe–membrane integrity assay, analysis of cytoplasmic membrane permeability, electron microscopy, monitoring of the effect of the mitochondrial membrane potential and DNA binding assay. CMF-EO effectively inhibited the growth of C. albicans, C. glabrata, C. tropicalis, S. cerevisiae, D. hansenii, and Z. parabailii with minimum inhibition concentrations of 31.25, 31.25, 62.5, 31.25, 15.625, and 15.625 μg/mL, respectively, and minimum fungicidal concentrations of 62.5, 125.0, 125.0, 62.5, 31.25, and 62.5 μg/mL, respectively. The elucidated antifungal mechanisms of CMF-EO included the destruction o...