Elucidation of the Regular Emission Mechanism of Volatile β-Ocimene with Anti-insect Function from Tea Plants ( Camellia sinensis ) Exposed to Herbivore Attack

作者：Guotai Jian, Yongxia Jia, Jianlong Li, Xiaochen Zhou, Yinyin Liao, Guangyi Dai, Ying Zhou, Jinchi Tang, Lanting Zeng · 发表于：Journal of Agricultural and Food Chemistry · 年份：2021 · DOI：10.1021/acs.jafc.1c03534 · 被引用次数：33 · 研究领域：Plant biochemistry and biosynthesis、Insect-Plant Interactions and Control、Insect and Pesticide Research

Herbivore-induced plant volatiles (HIPVs) play an important role in insect resistance. As a common HIPV in tea plants ( Camellia sinensis ), β-ocimene has shown anti-insect function in other plants. However, whether β-ocimene in tea plants also provides insect resistance, and its mechanism of synthesis and emission are unknown. In this study, β-ocimene was confirmed to interfere with tea geometrid growth via signaling. Light was identified as the key factor controlling regular emission of β-ocimene induced by the wounding from tea geometrids. β-Ocimene synthase (CsBOS1) was located in plastids and catalyzed β-ocimene formation in overexpressed tobacco. CsBOS1 expression in tea leaves attacked by tea geometrids showed a day-low and night-high variation pattern, while CsABCG expression involved in volatile emission showed the opposite pattern. These two genes might regulate the regular β-ocimene emission from tea plants induced by tea geometrid attack. This study advances the understanding on HIPV emission and signaling in tea plants.