Nicotinamide Riboside Alleviates Cardiac Dysfunction and Remodeling in Pressure Overload Cardiac Hypertrophy

作者：Sai Ma, Jing Feng, Xiuyu Lin, Jing Liu, Yi Tang, Shinan Nie, Jianbin Gong, Lei Wang · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2021 · DOI：10.1155/2021/5546867 · 被引用次数：38 · 研究领域：Sirtuins and Resveratrol in Medicine、Cardiac Ischemia and Reperfusion、Adipose Tissue and Metabolism

Background . Cardiac hypertrophy is a compensatory response to pressure overload, which eventually leads to heart failure. The current study explored the protective effect of nicotinamide riboside (NR), a NAD + booster that may be administered through the diet, on the occurrence of myocardial hypertrophy and revealed details of its underlying mechanism. Methods . Transverse aortic constriction (TAC) surgery was performed to establish a murine model of myocardial hypertrophy. Mice were randomly divided into four groups: sham, TAC, sham+NR, and TAC+NR. NR treatment was given daily by oral gavage. Cardiac structure and function were assessed using small animal echocardiography. Mitochondrial oxidative stress was evaluated by dihydroethidium (DHE) staining, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity. Levels of expression of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), IL‐1 β , TNF‐ α , and Sirtuin3 were measured by real‐time PCR and ELISA. Expression levels of Caspase‐1, Caspase‐1 pro, cleaved Gasdermin D (GSDMD), NLRP3, ASC, Sirtuin3, ac‐MnSOD, and total MnSOD were measured by Western blot. Results . Reductions in the heart/body mass ratio (HW/BW) and lung/body mass ratio (LW/BW) and in ANP, BNP, and LDH levels were observed in the TAC group on the administration of NR ( P < 0.05). Moreover, echocardiography data showed that cardiac dysfunction and structural changes caused by TAC were improved by NR treatment ( P < 0.05). ...