Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis

作者：Yongfeng Liu, Ran Han, Letian Zhou, Mingjie Luo, Lidong Zeng, Xiaochao Zhao, Yukun Ma, Zhiliang Zhou, Lei Sun · 发表于：BMC Genomics · 年份：2021 · DOI：10.1186/s12864-021-08150-8 · 被引用次数：41 · 研究领域：Genomics and Phylogenetic Studies、Cancer-related molecular mechanisms research、Molecular Biology Techniques and Applications

BACKGROUND: GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first report to compare the transcriptome and LncRNA sequencing data of the GenoLab M sequencer to NovaSeq 6000 platform in various types of analysis. RESULTS: We tested 16 libraries in three species using various library kits from different companies. We compared the data quality, genes expression, alternatively spliced (AS) events, single nucleotide polymorphism (SNP), and insertions-deletions (InDel) between two sequencing platforms. The data suggested that platforms have comparable sensitivity and accuracy in terms of quantification of gene expression levels with technical compatibility. CONCLUSIONS: Genolab M is a promising next-generation sequencing platform for transcriptomics and LncRNA studies with high performance at low costs.