Ultrafast, sensitive, and portable detection of COVID-19 IgG using flexible organic electrochemical transistors

作者：Hong Liu, Anneng Yang, Jiajun Song, Naixiang Wang, Puiyiu Lam, Yuenling Li, Hkw Law, Feng Yan · 发表于：Science Advances · 年份：2021 · DOI：10.1126/sciadv.abg8387 · 被引用次数：233 · 研究领域：SARS-CoV-2 detection and testing、Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection

The outbreak of COVID-19 and its continued spread have seriously threatened public health. Antibody testing is essential for infection diagnosis, seroepidemiological analysis, and vaccine evaluation. However, convenient, fast, and accurate antibody detection remains a challenge in this protracted battle. Here, we report an ultrafast, low-cost, label-free, and portable SARS-CoV-2 immunoglobulin G (IgG) detection platform based on organic electrochemical transistors (OECTs), which can be remotely controlled by a mobile phone. To enable faster detection, voltage pulses are applied on the gate electrode of the OECT to accelerate binding between the antibody and antigen. By optimizing ion concentrations and pH values of test solutions, we realize specific detection of SARS-CoV-2 IgG in several minutes with a detectable region from 10 fM to 100 nM, which encompasses the range of serum SARS-CoV-2 IgG levels in humans. These portable sensors show promise for use in diagnosis and prognosis of COVID-19.