The Fabrication of a Gellan Gum-Based Hydrogel Loaded With Magnesium Ions for the Synergistic Promotion of Skin Wound Healing

作者：Wenqiang Li, Xingling Jian, Yanfen Zou, Lin Wu, Haiyan Huang, Hui Li, Dandan Hu, Bo Yu · 发表于：Frontiers in Bioengineering and Biotechnology · 年份：2021 · DOI：10.3389/fbioe.2021.709679 · 被引用次数：37 · 研究领域：Wound Healing and Treatments、Hydrogels: synthesis, properties, applications、Tendon Structure and Treatment

To accelerate serious skin burn wound healing in a convenient manner, an interpenetrating network of hydrogel consisting of gellan gum and polyacrylamide was synthesized by chemical crosslinking and Mg 2+ ion immersion techniques. The prepared Mg 2+ @PAM/GG hydrogel was characterized by morphology, water vapor loss, swelling ratio, rheological properties, tensile mechanical, biocompatibility, and flow cytometry study. The results show that Mg 2+ @PAM/GG hydrogel’s mechanical strength could be enhanced by the dual network structure and physical crosslinking agent Mg 2+ ions. In addition, the tension strength of Mg 2+ @PAM/GG hydrogel is obviously increased from 86 to 392 kPa, the elongation at break increased from 84 to 231%, and crosslinking density N increased from 4.3 to 7.2 mol/m 3 compared with pure GG hydrogel. The cumulative release curve of Mg 2+ ions shows that the multiple release mechanism of Mg 2+ ions belong to non-Fick’s diffusion. Meanwhile, in vitro experiments show that Mg 2+ @PAM/GG double network hydrogel has favorable proliferation and an NF-κB pathway inhibition property for fibroblast cells. Finally, the healing effect of the Mg 2+ @PAM/GG was evaluated in a rat full-thickness burn model. The animal study demonstrates that Mg 2+ @PAM/GG could accelerate the healing efficiency in case of the sustained-released Mg 2+ ions in wound beds. Considering this excellent performance, this convenient prepared hydrogel has great potential as a commercial application ...