Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc

作者：Junchang Li, Yumei Jiang, Jıng Zhang, Yongjing Ni, Zhixin Jiao, Huijuan Li, Ting Wang, Peipei Zhang, Wenlong Guo, Lei Li, Hongjie Liu, Hairong Zhang, Qiao‐Yun Li, Jishan Niu · 发表于：PeerJ · 年份：2021 · DOI：10.7717/peerj.12221 · 被引用次数：35 · 研究领域：Plant Molecular Biology Research、Plant Reproductive Biology、Genetic Mapping and Diversity in Plants and Animals

Tillering ability is a key agronomy trait for wheat ( Triticum aestivum L.) production. Studies on a dwarf monoculm wheat mutant ( dmc ) showed that ARF11 played an important role in tillering of wheat. In this study, a total of 67 ARF family members were identified and clustered to two main classes with four subgroups based on their protein structures. The promoter regions of T. aestivum ARF ( TaARF ) genes contain a large number of cis -acting elements closely related to plant growth and development, and hormone response. The segmental duplication events occurred commonly and played a major role in the expansion of TaARFs . The gene collinearity degrees of the ARFs between wheat and other grasses, rice and maize, were significantly high. The evolution distances among TaARFs determine their expression profiles, such as homoeologous genes have similar expression profiles, like TaARF4-3A-1 , TaARF4-3A-2 and their homoeologous genes. The expression profiles of TaARFs in various tissues or organs indicated TaARF3 , TaARF4 , TaARF9 and TaARF22 and their homoeologous genes played basic roles during wheat development. TaARF4 , TaARF9 , TaARF12 , TaARF15 , TaARF17 , TaARF21 , TaARF25 and their homoeologous genes probably played basic roles in tiller development. qRT-PCR analyses of 20 representative TaARF genes revealed that the abnormal expressions of TaARF11 and TaARF14 were major causes constraining the tillering of dmc . Indole-3-acetic acid (IAA) contents in dmc were significan...