Effects of acute deltamethrin exposure on kidney transcriptome and intestinal microbiota in goldfish (Carassius auratus)

作者：Shun Zhou, Jing Fei Dong, Yongtao Liu, Qiuhong Yang, Ning Xu, Yibin Yang, Xiaohui Ai · 发表于：Ecotoxicology and Environmental Safety · 年份：2021 · DOI：10.1016/j.ecoenv.2021.112716 · 被引用次数：37 · 研究领域：Aquaculture disease management and microbiota、Environmental Toxicology and Ecotoxicology、Pharmaceutical and Antibiotic Environmental Impacts

As a widely used synthetic pyrethroid insecticide, deltamethrin (DM) causes serious health problems to aquatic organisms. However, the comprehensive understanding of the adverse effect of DM on aquatic organisms has received limited attention. In this study, goldfish (Carassius auratus) were exposed to 0 (control group), 0.2 and 2 µg/L DM for 96 h. The kidney transcriptome and intestinal microbiota were investigated. Comparative transcriptome analysis identified 270 and 711 differentially expressed genes (DEGs) in goldfish kidneys after exposure to 0.2 and 2 µg/L DM, respectively. KEGG pathway analysis revealed that the apoptosis pathway was markedly regulated and the regulation of programmed cell death was significantly enriched by the GO analysis. Several apoptosis-related genes including cathepsin L and cytochrome c were also detected. These results indicated that apoptosis occurred in the goldfish kidney after acute exposure to sublethal concentration of DM. Besides, some immune and drug metabolism-related DEGs were identified, indicating that exposure to DM caused immunotoxicity and metabolic disruption in goldfish. Additionally, 16 S rRNA gene sequencing analysis revealed a remarkable alteration in the composition of the intestinal microbial community of DM-treated goldfish. At the phylum level, the abundance of Proteobacteria, Firmicutes and Fusobacteria was increased, whereas the abundance of Bacteroidetes was reduced significantly after DM exposure. At the genus leve...