Blocking miR530 Improves Rice Resistance, Yield, and Maturity

作者：Yan Li, Liang‐Fang Wang, Sadam Hussain Bhutto, Xiaorong He, Xue‐Mei Yang, Xinhui Zhou, Xiaoyu Lin, Aisha Anum Rajput, Guo‐Bang Li, Jing‐Hao Zhao, Shixin Zhou, Yunpeng Ji, Mei Pu, He Wang, Zhi‐Xue Zhao, Yanyan Huang, Ji‐Wei Zhang, Peng Qin, Jing Fan, Wenming Wang · 发表于：Frontiers in Plant Science · 年份：2021 · DOI：10.3389/fpls.2021.729560 · 被引用次数：33 · 研究领域：Plant Molecular Biology Research、Plant Disease Resistance and Genetics、Plant Stress Responses and Tolerance

MicroRNAs fine-tune plant growth and resistance against multiple biotic and abiotic stresses. The trade-off between biomass and resistance can penalize crop yield. In this study, we have shown that rice miR530 regulates blast disease resistance, yield, and growth period. While the overexpression of miR530 results in compromised blast disease resistance, reduced grain yield, and late maturity, blocking miR530 using a target mimic (MIM530) leads to enhanced resistance, increased grain yield, and early maturity. Further study revealed that the accumulation of miR530 was decreased in both leaves and panicles along with the increase of age. Such expression patterns were accordant with the enhanced resistance from seedlings to adult plants, and the grain development from panicle formation to fully-filled seeds. Divergence analysis of miR530 precursor with upstream 1,000-bp promoter sequence in 11 rice species revealed that miR530 was diverse in Oryza sativa japonica and O. sativa indica group, which was consistent with the different accumulation of miR530 in japonica accessions and indica accessions. Altogether, our results indicate that miR530 coordinates rice resistance, yield, and maturity, thus providing a potential regulatory module for breeding programs aiming to improve yield and disease resistance.