Hyperspectral stimulated Raman scattering microscopy facilitates differentiation of low-grade and high-grade human prostate cancer

作者：Xun Chen, Sishan Cui, Shuai Yan, Shuo Zhang, Fan Yu, Yanqing Gong, Liqun Zhou, Ping Wang, Lin Yao, Shuhua Yue · 发表于：Journal of Physics D Applied Physics · 年份：2021 · DOI：10.1088/1361-6463/ac2175 · 被引用次数：17 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Spectroscopy and Chemometric Analyses、Molecular Biology Techniques and Applications

Abstract Histopathology currently acts as a gold standard for human prostate cancer (PCa) diagnosis. However, the subjective nature of histopathology leads to inevitable discordance among pathologists. Specifically, the inter-observer discordance could be up to 40% for the differentiation between Gleason score 6 (low-grade) and 7 (high-grade) of PCa. According to clinical guidelines, patients with high-grade PCa need to be actively treated, while patients with low-grade PCa could undergo active surveillance due to its slowly growing feature. Therefore, differentiating high-grade and low-grade PCa is an urgent clinical need. By integrating stimulated Raman scattering microscopy and confocal Raman spectroscopy, our previous study found the aberrant cholesteryl ester (CE) accumulation in human PCa tissues. However, no significant difference in CE accumulation between the low-grade and high-grade PCa was found, primarily because the previous study only analyzed the composition of manually selected lipid droplets (LDs) without quantitative analysis of the whole field of view. Here, we employed hyperspectral stimulated Raman scattering (HSRS) microscopy to test the hypothesis of CE as a marker for differentiation of low-grade and high-grade human PCa. First, lipid, lipofuscin, and protein were quantitatively mapped in human prostate tissues based on HSRS imaging of C–H vibrational region and multivariate curve resolution analysis. Then, within the lipid channel, CE percentage and u...