Molecular Characteristics of Human Adenovirus Type 3 Circulating in Parts of China During 2014–2018

作者：Yali Duan, Baoping Xu, Changchong Li, Yixiao Bao, Shuhua An, Yunlian Zhou, Aihuan Chen, Li Deng, Limin Ning, Yun Zhu, Wei Wang, Meng Zhang, Lili Xu, Xiangpeng Chen, Zhengde Xie · 发表于：Frontiers in Microbiology · 年份：2021 · DOI：10.3389/fmicb.2021.688661 · 被引用次数：22 · 研究领域：Virus-based gene therapy research、Viral gastroenteritis research and epidemiology、Respiratory viral infections research

Human adenoviruses (HAdVs) are important pathogens causing respiratory infections; 3.5-11% of childhood community-acquired pneumonia is associated with HAdV infection. Human adenovirus type 3 (HAdV-3), leading to severe morbidity and mortality, is one of the most prevalent genotype among adenoviruses responsible for acute respiratory infections (ARIs) in children in China. To identify the genetic variation of HAdV-3 in children with ARIs in China, a molecular epidemiological study was conducted. A total of 54 HAdV-3 isolated strains were obtained from children with ARIs in Beijing, Wenzhou, Shanghai, Shijiazhuang, Hangzhou, Guangzhou, and Changchun from 2014 to 2018. Thirty-two strains of which were selected for whole-genome sequencing, while the hexon, penton base, and fiber genes were sequenced for remaining strains. Bioinformatics analysis was performed on the obtained sequences. The phylogenetic analyses based on whole-genome sequences, major capsid protein genes (hexon, penton base, and fiber), and early genes (E1, E2, E3, and E4) showed that the HAdV-3 strains obtained in this study always clustered together with the reference strains from Chinese mainland, while the HAdV-3 prototype strain formed a cluster independently. Compared with the prototype strain, all strains possessed nine amino acid (AA) substitutions at neutralization antigenic epitopes of hexon. The homology models of the hexon protein of the HAdV-3 prototype and strain BJ20160214 showed that there was no ...