Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research

作者：Mario Rothbauer, Ruth A. Byrne, Silvia Schobesberger, Isabel Olmos Calvo, Anita Fischer, Eva I. Reihs, Sarah Spitz, Barbara Bachmann, Florian Sevelda, Johannes Holinka, Wolfgang Holnthoner, Heinz Redl, Stefan Toegel, Reinhard Windhager, Hans P. Kiener, Peter Ertl · 发表于：Lab on a Chip · 年份：2021 · DOI：10.1039/d1lc00130b · 被引用次数：88 · 研究领域：Rheumatoid Arthritis Research and Therapies、Cellular Mechanics and Interactions、Orthopedic Infections and Treatments

cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases.