Dynamic network biomarker analysis discovers IbNAC083 in the initiation and regulation of sweet potato root tuberization

作者：Shutao He, Hongxia Wang, Xiaomeng Hao, Yinliang Wu, Xiaofeng Bian, Minhao Yin, Yandi Zhang, Weijuan Fan, Hao Dai, Ling Yuan, Peng Zhang, Luonan Chen · 发表于：The Plant Journal · 年份：2021 · DOI：10.1111/tpj.15478 · 被引用次数：86 · 研究领域：Plant nutrient uptake and metabolism、Plant Molecular Biology Research、Genetic Mapping and Diversity in Plants and Animals

The initiation and development of storage roots (SRs) are intricately regulated by a transcriptional regulatory network. One key challenge is to accurately pinpoint the tipping point during the transition from pre-swelling to SRs and to identify the core regulators governing such a critical transition. To solve this problem, we performed a dynamic network biomarker (DNB) analysis of transcriptomic dynamics during root development in Ipomoea batatas (sweet potato). First, our analysis identified stage-specific expression patterns for a significant proportion (>9%) of the sweet potato genes and unraveled the chronology of events that happen at the early and later stages of root development. Then, the results showed that different root developmental stages can be depicted by co-expressed modules of sweet potato genes. Moreover, we identified the key components and transcriptional regulatory network that determine root development. Furthermore, through DNB analysis an early stage, with a root diameter of 3.5 mm, was identified as the critical period of SR swelling initiation, which is consistent with morphological and metabolic changes. In particular, we identified a NAM/ATAF/CUC (NAC) domain transcription factor, IbNAC083, as a core regulator of this initiation in the DNB-associated network. Further analyses and experiments showed that IbNAC083, along with its associated differentially expressed genes, induced dysfunction of metabolism processes, including the biosynthesis of li...