Biofilm properties in relation to treatment outcome in patients with first-time periprosthetic hip or knee joint infection

作者：Karin Svensson Malchau, Jonatan Tillander, Magdalena Zaborowska, Maria Hoffman, Íñigo Lasa, Peter Thomsen, Henrik Malchau, Ola Rolfson, Margarita Trobos · 发表于：Journal of Orthopaedic Translation · 年份：2021 · DOI：10.1016/j.jot.2021.05.008 · 被引用次数：70 · 研究领域：Orthopedic Infections and Treatments、Bacterial biofilms and quorum sensing、Orthopaedic implants and arthroplasty

BACKGROUND: Periprosthetic joint infections (PJI) are challenging complications following arthroplasty. Staphylococci are a frequent cause of PJI and known biofilm producers. Biofilm formation decreases antimicrobial susceptibility, thereby challenging favourable treatment outcomes. The aims of this study were to characterize the biofilm abilities and antimicrobial susceptibilities of staphylococci causing first-time PJI and correlate them to clinical outcome (infection resolution and recurrence). METHODS: Reoperations for PJI of the hip or knee between 1st January 2012 to 30th June 2015 performed at the Sahlgrenska University Hospital were identified in a local database. Medical records were reviewed and clinical parameters recorded for patients whose intraoperative bacterial isolates had been stored at the clinical laboratory. Staphylococcal strains isolated from reoperations due to first-time PJI were characterised by their ability to form biofilms using the microtiter plate test. Antimicrobial susceptibility of the strains was determined by minimum inhibitory concentration (MIC) when grown planktonically, and by minimum biofilm eradication concentration (MBEC) when grown as biofilms. MBEC determination was conducted using the Calgary biofilm device (CBD) and a custom-made antimicrobial susceptibility plate containing eight clinically relevant antimicrobial agents. RESULTS: The study group included 49 patients (70 bacterial strains) from first-time PJI, whereof 24 (49%) pa...