Establishment of Full-Length cDNA Clones and an Efficient Oral Infection Model for Feline Coronavirus in Cats

作者：Gang Wang, Guangli Hu, Rui Liang, Jiale Shi, Xiuxiu Qiu, Yiling Yang, Zhe Jiao, Yixi Chen, Zhou Shen, Mengxia Li, Yuejun Shi, Junfu Mao, Guiqing Peng · 发表于：Journal of Virology · 年份：2021 · DOI：10.1128/jvi.00745-21 · 被引用次数：25 · 研究领域：Animal Virus Infections Studies、Virus-based gene therapy research、Viral gastroenteritis research and epidemiology

Tissue- or cell culture-adapted feline infectious peritonitis virus (FIPV) usually loses pathogenicity. To develop a highly virulent FIPV, we constructed a field isolate type I FIPV full-length clone with the spike gene replaced by the 79-1146 spike gene, corresponding to a virus named rQS-79, which induces high mortality in adult cats. rQS-79 represents the first described reverse genetics system for highly pathogenic FCoV.