Protective Role of 4‐Octyl Itaconate in Murine LPS/D‐GalN‐Induced Acute Liver Failure via Inhibiting Inflammation, Oxidative Stress, and Apoptosis

作者：Ruidong Li, Wenchang Yang, Yuping Yin, Peng Zhang, Yaxin Wang, Kaixiong Tao · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2021 · DOI：10.1155/2021/9932099 · 被引用次数：56 · 研究领域：Pharmacological Effects of Natural Compounds、Aldose Reductase and Taurine、Biochemical effects in animals

Oxidative stress, inflammation, and apoptosis are crucial in the pathogenesis of acute liver failure (ALF). 4‐Octyl itaconate (OI) showed antioxidative and anti‐inflammatory properties in many disease models. However, its role in lipopolysaccharide‐ (LPS‐)/D‐galactosamine‐ (D‐GalN‐) induced ALF is still not investigated. Here, we established an ALF murine model induced by LPS/D‐GalN administration. And we found that OI improved survival rate in the murine ALF model. Our results also showed that OI alleviated LPS/D‐GalN‐induced hepatic histopathological injury and reduced the serum activities of alanine transaminase and aspartate transaminase. Moreover, OI reduced serum levels of proinflammatory cytokines such as monocyte chemotactic protein‐1, tumor necrosis factors‐ α , and interlukin‐6. Additionally, OI mitigated oxidative stress and alleviated lipid peroxidation in a murine model of ALF. This was evaluated by a reduction of thiobarbituric acid reactive substances (TBARS) in liver tissues. In addition, OI increased the ratio of reduced glutathione/oxidized glutathione and the activities of antioxidant enzymes including catalase and superoxide dismutase. Moreover, the apoptosis of hepatocytes in the liver was inhibited by OI. Furthermore, we found that OI inhibited LPS‐induced nuclear translocation and activation of factor‐kappa B (NF‐ κ B) p65 in macrophages which could be inhibited by OI‐induced activation of nuclear factor erythroid‐2‐related factor (Nrf2) signaling. Addi...