Genotype distribution of HCV among the populations of IDUs and non-IDUs in Hainan

作者：Jiao Wang, Ming Liu, Biao Wu, Hui Li, MO Shaoxiong, Wang Zhaoqian, LU Qiujuan, Chen Jinguan, Shuli Chen, Lin Feng, Tao Wu · 发表于：Zhongguo redai yixue · 年份：2019 · DOI：10.13604/j.cnki.46-1064/r.2019.11.06 · 研究领域：Hepatitis C virus research、Hepatitis B Virus Studies、Alcohol Consumption and Health Effects

Objective To define the main gene subtypes and HCV-Ab, HCV-RNA of hepatitis C virus (HCV) infectors among inject drug users (IDUs) and non-inject drug users (non-IDUs) in Hainan Province. Methods The HCV-RNA(s) of 581 samples, that were from Methadone Maintenance Treatment Clinics, drug treatment center and Hainan Provincial People’s Hospital Infection Department, were extracted, amplified in the HCV Core-E1 region by RT-PCR and nested-PCR, and determine the HCV genotype distribution. Based on whether or not absence of intravenous drug abuse, the subjects were divided into IDUs group and non-IDUs group, statistical analysis was made on the demographic data. Results Among 802 methadone maintenance treatment outpatients and drug addicts in Hainan Province, 309 were HCV-Ab positive, with the HCV-Ab positive rate of 38.53%, and 215 were HCV-RNA positive, with the positive rate of 69.58%. And 251 patients tested HCV-RNA positive in Hainan People’s Hospital, 466 patients tested positive for HCV-RNA. Of the 581 HCV-Ab positive cases, 466 of them HCV-RNA positive cases.And 448 out of the 466 sampleswere successfully sequenced and genotyped as follows,the subtypes and proportion among 320 IDUs were found as 6a (58.75%) in mainly, and followed by 3b (16.88%), 3a (15.63%), 1a (4.38%), 1b (2.81%) and non-6a (0.93%); 128 non-IDUs were 1b (30.47%), 6a (28.12%), 3b (10.16%), 3a (3.91%), 1a (7.03%) and non-6a (14.84%), the difference was statistically significant ( P <0.01). Conclusion The s...