Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease

作者：Jing Shao, Yan Jin, Chunhong Shao, Hui Fan, Xiaorui Wang, Guang Yang · 发表于：Cellular & Molecular Biology Letters · 年份：2021 · DOI：10.1186/s11658-021-00280-x · 被引用次数：43 · 研究领域：Extracellular vesicles in disease、Pregnancy and Medication Impact、COVID-19 Impact on Reproduction

BACKGROUND: Inflammatory bowel disease (IBD) is a kind of intestinal immune dysfunction disease, and its occurrence and prevalence are on the rise worldwide. As a chronic gastrointestinal disease, its pathogenesis is still unknown. Exosomes are vesicles in various body fluids that carry a variety of substances. They can mediate intercellular communication and long-distance transport of multiple media. In this study, we investigated the protein profile of serum exosomes from healthy people and IBD patients to explore a new serological biomarker for IBD. METHODS: Initially, exosomes were extracted from serum samples, and the proteins within the exosomes were identified by label-free liquid chromatography/mass spectrometry (LC-MS/MS). Western blot and ELISA were used to assess the identified protein. To further analyze the target protein, an acute colitis mouse model was established, and exosomes in colonic tissue and serum were extracted to investigate the protein in them. RESULTS: Firstly, serum exosomes were extracted from samples, and proteins in exosomes were identified by LC-MS/MS. Through statistical analysis, we identified 633 proteins. Among these proteins, pregnancy zone protein (PZP) showed a marked difference between patients with IBD and healthy people, in that its expression level was much higher in the IBD patients This exosomal protein was associated with immunosuppressive effects. Also, the level of PZP in colon tissue exosomes and serum exosomes of acute coliti...