Gadolinium chloride pre‑treatment reduces the inflammatory response and preserves intestinal barrier function in a rat model of sepsis

作者：Yan Zhao, Shun Zhang, Hai Zhao, Hui Qin, Fang Wu, Jie Zhang, Yu Zhang, Xiao Liu, Su Liang, Hui Zhang, Jiang Wu, Zheng Zhao, Hong Wang, Meng Shao, Jing Liu, Dong Jiang, Wan Zhang · 发表于：Experimental and Therapeutic Medicine · 年份：2021 · DOI：10.3892/etm.2021.10577 · 被引用次数：2 · 研究领域：Barrier Structure and Function Studies、Immune Response and Inflammation、Enhanced Recovery After Surgery

The inflammatory response is closely associated with sepsis occurrence and progression. Damage to the function of the intestinal mucosal barrier is considered to be the ῾initiation factor᾿ for the development of multiple organ dysfunction syndrome, which is the most severe progression of sepsis. The aim of the present study was to investigate whether gadolinium chloride (GdCl 3 ) could alleviate the systemic inflammatory response and protect the function of the intestinal mucosal barrier in a rat model of sepsis. The mechanism underlying this protective effect was also explored. Sprague‑Dawley rats were divided into four groups: Sham, sham + GdCl 3 , cecal ligation and puncture (CLP; a model of sepsis) and CLP + GdCl 3 . In each group, blood was collected from the abdominal aorta, and intestinal tissue was collected after 6, 12 and 24 h of successful modeling. Levels of tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑1β were determined using ELISA. Western blot analysis was used to determine levels of occludin, tight junction protein ZO‑1 (ZO‑1), myosin light chain kinase 3 (MLCK), NF‑κB and caspase‑3 in intestinal tissues. Hematoxylin‑eosin staining was used to observe the degree of damage to intestinal tissue. The results indicated that in CLP sepsis model rats treated with GdCl 3 , the release of systemic and intestinal pro‑inflammatory factors was reduced and tissue damage was alleviated when compared with untreated CLP rats. Additionally, the expression of occludin an...