Plant developmental stage drives the differentiation in ecological role of the maize microbiome

作者：Chao Xiong, Brajesh K. Singh, Ji‐Zheng He, Yan-Lai Han, Peipei Li, Li-Hua Wan, Guo-Zhong Meng, Si‐Yi Liu, Juntao Wang, Chuanfa Wu, An‐Hui Ge, Limei Zhang · 发表于：Microbiome · 年份：2021 · DOI：10.1186/s40168-021-01118-6 · 被引用次数：452 · 研究领域：Plant-Microbe Interactions and Immunity、Mycorrhizal Fungi and Plant Interactions、Gut microbiota and health

BACKGROUND: Plants live with diverse microbial communities which profoundly affect multiple facets of host performance, but if and how host development impacts the assembly, functions and microbial interactions of crop microbiomes are poorly understood. Here we examined both bacterial and fungal communities across soils, epiphytic and endophytic niches of leaf and root, and plastic leaf of fake plant (representing environment-originating microbes) at three developmental stages of maize at two contrasting sites, and further explored the potential function of phylloplane microbiomes based on metagenomics. RESULTS: Our results suggested that plant developmental stage had a much stronger influence on the microbial diversity, composition and interkingdom networks in plant compartments than in soils, with the strongest effect in the phylloplane. Phylloplane microbiomes were co-shaped by both plant growth and seasonal environmental factors, with the air (represented by fake plants) as its important source. Further, we found that bacterial communities in plant compartments were more strongly driven by deterministic processes at the early stage but a similar pattern was for fungal communities at the late stage. Moreover, bacterial taxa played a more important role in microbial interkingdom network and crop yield prediction at the early stage, while fungal taxa did so at the late stage. Metagenomic analyses further indicated that phylloplane microbiomes possessed higher functional dive...