N6-Methyladenosine Related Long Non-Coding RNAs and Immune Cell Infiltration in the Tumor Microenvironment of Gastric Cancer

作者：Zhong Lin Yu, Zheng Zhu · 发表于：Biological Procedures Online · 年份：2021 · DOI：10.1186/s12575-021-00152-w · 被引用次数：15 · 研究领域：RNA modifications and cancer、Cancer-related molecular mechanisms research、Ferroptosis and cancer prognosis

AIM: To illustrate the influence of N6-methyladenosine long non-coding RNAs and immune cell infiltration in gastric cancer. METHODS: We downloaded workflow-type data and clinical data from The Cancer Genome Atlas project. The relationship of lncRNA and m6A was identified. Kyoto Encyclopedia of Genes and Genomes gene expression enrichment analysis was performed. Lasso regression was utilized to construct a prognostic model. Survival analysis to explore the relationship between m6A lncRNA and clinical survival data. Differential analysis of the tumor microenvironment and immune correlation analysis to determine immune cell infiltration levels and their correlation with clinical prognosis. RESULTS: Co-expression analysis indicated that lncRNA expression was associated closely with m6A. m6A-lncRNAs were partially highly expressed in tumor tissue and could be used in a prognostic model to predict GC prognosis, independent of other clinical characteristics. "ADIPPOCYTOKINE SIGNALING PATHWAY" was most significantly enriched according to GSEA. ACBD3-AS1 was overexpressed in tumor tissue. Naïve B cell, Plasma cells, resting CD4 memory T cell were highly infiltrated tissues in cluster 2, while Macrophages M2, resting Mast cells, Monocytes, regulates T cells were lowly in cluster 1. All related scores were higher in cluster 2, indicating a lower purity of tumor cells and higher density of immune-related cells in the tumor microenvironment. CONCLUSION: m6A lncRNA is closely related to th...