Maternal supply of cysteamine alleviates oxidative stress and enhances angiogenesis in porcine placenta

作者：Shuangbo Huang, Zifang Wu, Zihao Huang, Xiangyu Hao, Longmiao Zhang, Cheng‐Jun Hu, Jianfu Wei, Jinping Deng, Chengquan Tan · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2021 · DOI：10.1186/s40104-021-00609-8 · 被引用次数：43 · 研究领域：Biomedical Research and Pathophysiology、Pregnancy and preeclampsia studies、Sulfur Compounds in Biology

Abstract Background Oxidative stress in placenta is associated with the occurrence of adverse pregnancy outcomes in sow, but there are few satisfactory treatment strategies for these conditions. This study investigated the potential of cysteamine (CS) as an antioxidant protectant for regulating the reproductive performance, redox status, and placental angiogenesis of sows. Methods The placental oxidative stress status and vascular density of piglets with different birth weights: < 1.0 kg (low birth weight, LBW) and 1.4–1.6 kg (normal birth weight, NBW) were evaluated, followed by allotting 84 sows to four treatments ( n = 21) and feeding them with a basal diet supplemented with 0, 100, 300, or 500 mg/kg of CS from d 85 of gestation to d 21 of lactation, respectively. Placenta, serum, and colostrum samples of sows or piglets were collected, and the characteristics of sows and piglets were recorded. Furthermore, the in vivo results were validated using porcine vascular endothelial cells (PVECs). Results Compared with the NBW placentae, the LBW placentae showed increased oxidative damage and were vulnerable to angiogenesis impairment. Particularly, H 2 O 2 -induced oxidative stress prompted intracellular reactive oxygen species generation and inhibited the tube formation and migration of PVECs as well as the expression of vascular endothelial growth factor-A (VEGF-A) in vitro . However, dietary CS supplementation can alleviate oxidative stress and improve the reproductive per...