Metagenomic analysis revealed the potential role of gut microbiome in gout

作者：Yong‐Liang Chu, Silong Sun, Yufen Huang, Qiang Gao, Xuefeng Xie, Peng Wang, Junxia Li, Lifeng Liang, Xiaohong He, Yiqi Jiang, Maojie Wang, Jianhua Yang, Xiumin Chen, Chu Zhou, Yue Zhao, Fen Ding, Yi Zhang, Xiaodong Wu, Xueyuan Bai, Jiaqi Wu, Xia Wei, Xianghong Chen, Zhen Yue, Xiaodong Fang, Qingchun Huang, Zhang Wang, Runyue Huang · 发表于：npj Biofilms and Microbiomes · 年份：2021 · DOI：10.1038/s41522-021-00235-2 · 被引用次数：238 · 研究领域：Gout, Hyperuricemia, Uric Acid、Gut microbiota and health、Liver Disease Diagnosis and Treatment

Emerging evidence indicates an association between gut microbiome and arthritis diseases including gout. However, how and which gut bacteria affect host urate degradation and inflammation in gout remains unclear. Here we performed a metagenome analysis on 307 fecal samples from 102 gout patients and 86 healthy controls. Gout metagenomes significantly differed from those of healthy controls. The relative abundances of Prevotella, Fusobacterium, and Bacteroides were increased in gout, whereas those of Enterobacteriaceae and butyrate-producing species were decreased. Functionally, gout patients had greater abundances for genes in fructose, mannose metabolism and lipid A biosynthesis, and lower for genes in urate degradation and short chain fatty acid production. A three-pronged association between metagenomic species, functions and clinical parameters revealed that decreased abundances of species in Enterobacteriaceae were associated with reduced amino acid metabolism and environmental sensing, which together contribute to increased serum uric acid and C-reactive protein levels in gout. A random forest classifier based on three gut microbial genes showed high predictivity for gout in both discovery and validation cohorts (0.91 and 0.80 accuracy), with high specificity in the context of other chronic disorders. Longitudinal analysis showed that uric-acid-lowering and anti-inflammatory drugs partially restored gut microbiota after 24-week treatment. Comparative analysis with obesi...