Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats

作者：Panpan Zheng, Jiyu Kang, Entong Xing, Bin Zheng, Xueyao Wang, Huacheng Zhou · 发表于：Frontiers in Physiology · 年份：2021 · DOI：10.3389/fphys.2021.699344 · 被引用次数：29 · 研究领域：Hydrogen's biological and therapeutic effects、Transplantation: Methods and Outcomes、Organ Transplantation Techniques and Outcomes

Background: Lung inflation with hydrogen is an effective method to protect donor lungs from lung ischemia-reperfusion injury (IRI). This study aimed to examine the effect of lung inflation with 3% hydrogen during the cold ischemia phase on pyroptosis in lung grafts of rats. Methods: Adult male Wistar rats were randomly divided into the sham group, the control group, the oxygen (O 2 ) group, and the hydrogen (H 2 ) group. The sham group underwent thoracotomy but no lung transplantation. In the control group, the donor lungs were deflated for 2 h. In the O 2 and H 2 groups, the donor lungs were inflated with 40% O 2 + 60% N 2 and 3% H 2 + 40% O 2 + 57% N 2 , respectively, at 10 ml/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. Two hours after orthotopic lung transplantation, the recipients were euthanized. Results: Compared with the control group, the O 2 and H 2 groups improved oxygenation indices, decreases the inflammatory response and oxidative stress, reduced lung injury, and improved pressure-volume (P-V) curves. H 2 had a better protective effect than O 2 . Furthermore, the levels of the pyroptosis-related proteins selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase (caspase)-1 p20, and the N-terminal of gasdermin D (GSDMD-N) were decreased in the H 2 group. Conclusion: Lung inflation with 3% hydrogen during the cold ischemia phase inhibited the inflammatory respon...