Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

作者：Juanjuan Xu, Yuanpei Li, Haojie Wang, Mengxi Zhu, Wenpo Feng, Gaofeng Liang · 发表于：International Journal of Nanomedicine · 年份：2021 · DOI：10.2147/ijn.s315839 · 被引用次数：106 · 研究领域：Antimicrobial Peptides and Activities、Bacterial biofilms and quorum sensing、Antimicrobial agents and applications

Background: The biofilms could protect bacteria from antibiotics and promote the production of drug-resistant strains, making the bacteria more difficult to be eradicated. Thus, we developed an [email protected] @AgNPs nanocomposite, which is formed by modifying silver nanoparticles (AgNPs) with antimicrobial peptides (AMP) modified nanocomposite to destroy biofilm in this study. Methods: The [email protected] @AgNPs nanocomposite was prepared with polymerization method and characterized by using ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). The antibacterial effects of the nanocomposite were investigated by using agar diffusion method and minimum inhibitory concentration (MIC) test. The quantitative analysis of the biofilm formation by the nanocomposite was conducted using crystal violet staining and confocal laser scanning microscope (CLSM). Results: The DLS and TEM analysis showed it was a spherical nanocomposite with 200 nm size and well dispersed . The results of UV-vis and FT-IR confirmed the presence of AMP and AgNPs. The nanocomposite had an excellent biocompatibility at 100 μg/mL. And the [email protected] @AgNPs nanocomposite showed superior antimicrobial activity against both Gram-negative ( E. coli, P. aeruginosa ) and Gram-positive ( S. aureus ) bacteria than AgNPs or AMP. Importantly, the mRNA expression of biofilm-related genes were decrease...