DNA Methylation Signature of Epileptic Encephalopathy-Related Pathogenic Genes Encoding Ion Channels in Temporal Lobe Epilepsy

作者：Hua Tao, Zengqiang Chen, Jianhao Wu, Jun Chen, Yusen Chen, Jiawu Fu, Chaowen Sun, Haihong Zhou, Wangtao Zhong, Xu Zhou, Keshen Li · 发表于：Frontiers in Neurology · 年份：2021 · DOI：10.3389/fneur.2021.692412 · 被引用次数：15 · 研究领域：Epilepsy research and treatment、Neuroscience and Neuropharmacology Research、Genetics and Neurodevelopmental Disorders

Epilepsy is characterized by highly abnormal synchronous discharge of brain neurons, and ion channels are fundamental in the generation and modulation of neural excitability. Considering that abnormal methylation can either activate or repress genes, this study was designed to explore the DNA methylation signature of pathogenic genes encoding ion channels in temporal lobe epilepsy (TLE). In total, 38 TLE patients and 38 healthy controls were enrolled in the study, and genomic DNA and total protein of the lymphocytes were extracted from peripheral blood samples to assess methylation and protein levels. The DNA methylation levels of all 12 genes examined were significantly lower in the TLE group than in the control group. After false-positive correction, 83.3% (10/12) of these genes, namely, gamma-aminobutyric acid type A receptor subunit beta1 (GABRB1) , gamma-aminobutyric acid type A receptor subunit beta2 (GABRB2) , gamma-aminobutyric acid type A receptor subunit beta1 (GABRB3) , glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) , glutamate ionotropic receptor NMDA type subunit 2A (GRIN2A) , glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B) , hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1) , potassium voltage-gated channel subfamily A member 2 (KCNA2) , potassium voltage-gated channel subfamily B member 1 (KCNB1) , and potassium sodium-activated channel subfamily T member 1 ( KCNT1) , were still differentially expressed. Among...