Logic‐Gated Proximity Aptasensing for Cell‐Surface Real‐Time Monitoring of Apoptosis

作者：Jiao Zheng, Qiwei Wang, Lin Shi, Pai Peng, Lili Shi, Tao Li · 发表于：Angewandte Chemie International Edition · 年份：2021 · DOI：10.1002/anie.202106651 · 被引用次数：80 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、Nanoplatforms for cancer theranostics

Abstract In nature, intact apoptotic cells release ATP as a signaling molecule to trigger prompt phagocytic clearance, even at the earliest stage of apoptosis. Inspired by this, here we introduce a straightforward strategy for real‐time monitoring ATP exocytosis and drug‐stimulated apoptosis in the cancer cell surroundings. Triplex‐boosted G‐quadruplexes (tb‐G4s) responding to cell environmental factors (H + and K + ) are engineered to construct a DNA logic‐gated nanoplatform for proximity ATP aptasensing on the cell surface. It enables the real‐time monitoring of cell apoptosis by capturing released endogenous ATP during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug‐induced apoptosis and therapeutic efficacy.