Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

作者：Jonathan Tyson, Kevin Hu, Shuai Zheng, P. Kidd, Neville Dadina, Ling Chu, Derek Toomre, Joerg Bewersdorf, Alanna Schepartz · 发表于：ACS Central Science · 年份：2021 · DOI：10.1021/acscentsci.1c00670 · 被引用次数：64 · 研究领域：Advanced Fluorescence Microscopy Techniques、Advanced Electron Microscopy Techniques and Applications、Photosynthetic Processes and Mechanisms

High Resolution Image Download MS PowerPoint Slide New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale 676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale 676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.