Altered Actinobacteria and Firmicutes Phylum Associated Epitopes in Patients With Parkinson’s Disease

作者：Zhuo Li, Gang Lü, Zhe Li, Bin Wu, Enli Luo, Xinmin Qiu, Jianwen Guo, Zhangyong Xia, Chunye Zheng, Qiaozhen Su, Yan Zeng, Wai‐Yee Chan, Xianwei Su, Qiaodi Cai, Yanjuan Xu, Yingjun Chen, Mingbang Wang, Wai Sang Poon, Xiaodong Luo · 发表于：Frontiers in Immunology · 年份：2021 · DOI：10.3389/fimmu.2021.632482 · 被引用次数：68 · 研究领域：Gut microbiota and health、Dysphagia Assessment and Management、Mycobacterium research and diagnosis

Recent evidence suggests that inflammation was participated in the pathogenesis of PD, thus, to understand the potential mechanism of gut microbiota in the pathogenesis of Parkinson’s disease (PD), we performed a metagenomic analysis of fecal samples from PD patient and controls. Using a two-stage metagenome-wide association strategy, fecal DNA samples from 69 PD patients and 244 controls in three groups (comprising 66 spouses, 97 age-matched, and 81 normal samples, respectively) were analyzed, and differences between candidate gut microbiota and microbiota-associated epitopes (MEs) were compared. In the study, 27 candidate bacterial biomarkers and twenty-eight candidate epitope peptides were significantly different between the PD patients and control groups. Further, enriched 4 and 13 MEs in PD were positively associated with abnormal inflammatory indicators [neutrophil percentage (NEUT.1), monocyte count/percentage (MONO/MONO.1), white blood cell count (WBC)] and five candidate bacterial biomarkers (c_Actinobacteria, f_Bifidobacteriaceae, g_Bifidobacterium , o_Bifidobacteriales, p_Actinobacteria) from Actinobacteria phylum, and they were also positively associated with histidine degradation and proline biosynthesis pathways, respectively. Additionally, enriched 2 MEs and 1 ME in PD were positively associated with above inflammatory indicators and two bacteria (f_Lactobacillaceae, g_Lactobacillus ) from Firmicutes phylum, and they were also positively associated with pyruvat...