Adjacent Cell Marker Lateral Spillover Compensation and Reinforcement for Multiplexed Images

作者：Yunhao Bai, Bokai Zhu, Xavier Rovira Clavé, Han M. Chen, Maxim Markovic, Chi Ngai Chan, Tung-Hung Su, David Robert McIlwain, Jacob D. Estes, Leeat Keren, Garry P. Nolan, Sizun Jiang · 发表于：Frontiers in Immunology · 年份：2021 · DOI：10.3389/fimmu.2021.652631 · 被引用次数：64 · 研究领域：Cell Image Analysis Techniques、Single-cell and spatial transcriptomics、Image Processing Techniques and Applications

Multiplex imaging technologies are now routinely capable of measuring more than 40 antibody-labeled parameters in single cells. However, lateral spillage of signals in densely packed tissues presents an obstacle to the assignment of high-dimensional spatial features to individual cells for accurate cell-type annotation. We devised a method to correct for lateral spillage of cell surface markers between adjacent cells termed REinforcement Dynamic Spillover EliminAtion (REDSEA). The use of REDSEA decreased contaminating signals from neighboring cells. It improved the recovery of marker signals across both isotopic (i.e., Multiplexed Ion Beam Imaging) and immunofluorescent (i.e., Cyclic Immunofluorescence) multiplexed images resulting in a marked improvement in cell-type classification.