Fluorescent Protein Expression as a Proxy for Bacterial Fitness in a High-Throughput Assay

作者：R. Schlechter, Evan J. Kear, Daniela M. Remus, Mitja N. P. Remus‐Emsermann · 发表于：Applied and Environmental Microbiology · 年份：2021 · DOI：10.1128/aem.00982-21 · 被引用次数：19 · 研究领域：Advanced Fluorescence Microscopy Techniques、Bacterial Genetics and Biotechnology、Cell Image Analysis Techniques

We expand on an important limitation of the concept of measuring bacterial growth, which is classically limited to one strain at a time. By adopting our approach, it is possible to measure the growth of several bacterial strains simultaneously with high temporal resolution and in a high-throughput manner. This is important to investigate bacterial interactions, such as competition and facilitation.