An Overview of 24 Years of Molecular Phylogenetic Studies in Phallales (Basidiomycota) With Notes on Systematics, Geographic Distribution, Lifestyle, and Edibility

作者：Gislaine Cristina de Souza Melanda, Alexandre G. S. Silva-Filho, Alexandre Rafael Lenz, Nelson Menolli, Alexandro de Andrade de Lima, Renato Juciano Ferreira, Nathalia Mendonça de ASSIS, Tiara Sousa Cabral, María P. Martín, Iuri Goulart Baseia · 发表于：Frontiers in Microbiology · 年份：2021 · DOI：10.3389/fmicb.2021.689374 · 被引用次数：18 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Lichen and fungal ecology、Yeasts and Rust Fungi Studies

The order Phallales ( Basidiomycota ) is represented by gasteroid fungi with expanded and sequestrate basidiomata, known as stinkhorns and false truffles. In phalloids, the first DNA sequence was published in 1997, and after that, some studies aimed to resolve phylogenetic conflicts and propose new species based on DNA markers; however, the number of families and genera in the order still generates controversies among researchers. Thus, this work aims to provide an overview of Phallales diversity represented by selected DNA markers available in public databases. We retrieved Phallales sequences from DNA databases (GenBank and UNITE) of seven markers: ITS (internal transcribed spacer), nuc-LSU (nuclear large subunit rDNA), nuc-SSU (nuclear small subunit rDNA), mt-SSU (mitochondrial small subunit rDNA), ATP 6 (ATPase subunit 6), RPB 2 (nuclear protein-coding second largest subunit of RNA polymerase), and TEF 1-α (translation elongation factor subunit 1α). To compose our final dataset, all ITS sequences retrieved were subjected to BLASTn searches to identify additional ITS sequences not classified as Phallales . Phylogenetic analyses based on Bayesian and maximum likelihood approaches using single and combined markers were conducted. All ITS sequences were clustered with a cutoff of 98% in order to maximize the number of species hypotheses. The geographic origin of sequences was retrieved, as well as additional information on species lifestyle and edibility. We obtained a total ...