Function of Polyamines in Regulating Cell Cycle Progression of Cultured Silkworm Cells

作者：Chang Li, Zhiqing Li, Hao Guo, Wenchang Zhang, Weiqun Lan, Jue Wang, Guanwang Shen, Qingyou Xia, Ping Zhao · 发表于：Insects · 年份：2021 · DOI：10.3390/insects12070624 · 被引用次数：14 · 研究领域：Polyamine Metabolism and Applications、Silk-based biomaterials and applications、Studies on Chitinases and Chitosanases

BACKGROUND: Putrescine, spermidine, and spermine are polyamines that are ubiquitously distributed in prokaryotic and eukaryotic cells, which play important roles in cell proliferation and differentiation. METHODS: We investigated the expression profiles of polyamine pathway genes by qRT-PCR in different tissues of the lepidopteran silkworm. The polyamine levels in cultured silkworm cells were measured by HPLC. Spermidine and polyamine biosynthetic inhibitors were used for treating the cultured silkworm cells in order to clarify their effects on cell cycle progression. RESULTS: We identified the anabolic and catabolic enzymes that are involved in the polyamine biosynthetic pathway in silkworm. Transcriptional expression showed at least seven genes that were expressed in different silkworm tissues. Treatments of the cultured silkworm cells with spermidine or inhibitor mixtures of DFMO and MGBG induced or inhibited the expression of cell cycle-related genes, respectively, and thus led to changed progression of the cell cycle. CONCLUSIONS: The present study is the first to identify the polyamine pathway genes and to demonstrate the roles of polyamines on cell cycle progression via regulation of the expression of cell cycle genes in silkworm.