Versatile Double-Beam Confocal Laser System Combined with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Photodissociation Mass Spectrometry and Spectroscopy

作者：Kailin Zhang, Yingying Shi, Mengying Du, Yicheng Xu, Yan Wang, Xianglei Kong · 发表于：Analytical Chemistry · 年份：2021 · DOI：10.1021/acs.analchem.1c00248 · 被引用次数：10 · 研究领域：Mass Spectrometry Techniques and Applications、Analytical Chemistry and Chromatography、Photoreceptor and optogenetics research

Both infrared multiphoton dissociation (IRMPD) and ultraviolet photodissociation (UVPD) play important roles in tandem mass spectrometry and the action spectroscopy of organic and biological molecules. A flexible combination of the two methods may provide researchers with more versatile and powerful ion activation/dissociation choices for structural characterization and spectroscopic studies. Here, we report the integration of two tunable lasers with a Fourier transform ion cyclotron resonance mass spectrometer in a confocal mode, which offers multiple capabilities for photon activation/dissociation experiments. The two overlapped beams can be introduced into the cell individually, sequentially, or simultaneously, providing highly flexible and diverse activation schemes. The setup can also measure the UVPD or IRMPD action spectra of fragment ions generated by previous photon dissociation processes. In addition, the multistage tandem-in-time mass spectrometry performance up to MS 4, including three different activation methods in a single cell, has also been demonstrated.