Evidence for the presence of a reversible Ca2+-dependent pore activated by oxidative stress in heart mitochondria

作者：M Crompton, Andreas COSTI, L. Hayat · 发表于：Biochemical Journal · 年份：1987 · DOI：10.1042/bj2450915 · 被引用次数：337 · 研究领域：Mitochondrial Function and Pathology、Electron Spin Resonance Studies、Lipid Membrane Structure and Behavior

Rat heart mitochondria became permeabilized to sucrose when incubated with 100 nmol of Ca2+/mg of protein in the presence of Pi. Ca2+ chelation with EGTA restored impermeability to sucrose, which became entrapped in the matrix space. t-Butylhydroperoxide markedly promoted permeabilization in the presence of Ca2+ but not in its absence, and Ca2+-plus-t-butylhydroperoxide-induced permeabilization was reversed by EGTA. The data suggest that Ca2+ and oxidative stress synergistically promote the reversible opening of an inner membrane pore.